Using the Organ Bath: 4 – Completing a full concentration-response curve

In order to obtain a complete concentration-response curve, tissue contractions are measured using the lowest concentration of drug first, and then we continue in ascending order to higher concentrations of drug. It is possible that no observable contraction can be seen at the lowest drug concentrations. However, as drug concentrations are increased, obvious contraction will be seen, such as in this example. Remember that each drug concentration is tested 3 times, or in triplicate, and with wash steps in between, before moving on to the next highest drug concentration.

Video 4/4 - Using the Organ Bath

Video Tutorials by Dr Matthew Cheesman,

School of Biomedical Sciences, University of Queensland, Brisbane Queensland 4072, Australia