Using the Organ Bath: 3 – Washing the organ bath
Description
These clamps allow the flow of buffer in and out of the organ bath. One of them is located at the bottom, which releases the chamber buffer contents, while an upper clamp allows fresh buffer to enter. Just before starting a wash step, be sure to include a wash label on the computer first. Squeeze the lower clamp to release the stored buffer. Then, squeeze the upper clamp to re-fill the chamber. Repeat this three times in total. Finally, lift the overflow tube to return the chamber volume to 25 ml. Wash steps cause fluctuations in the tension on the string that connects the tissue, and this can be seen on the trace. This is why it is important to label the computer with the wash steps.
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Attribution:
Matthew Cheesman, The University of Queensland