Using the Organ Bath: 2 – Adding drugs and recording contractions

As an example, let’s add acetylcholine to a final concentration of 1 uM in the organ bath. Use the bar at the bottom of the trace on the computer to label the experiment with both the concentration and name of the drug. Select the correct stock concentration of drug, and set your pipettor to the appropriate volume. Withdraw the drug from the eppendorf tube, and add it to the chamber just below the surface of the buffer and into the Carbogen bubbles. At the same time, press Enter on your computer to mark the addition. If a contraction is observed, and once you are confident that a plateau is obtained, you can then wash the organ bath.

Video 2/4 - Using the Organ Bath

Video Tutorials by Dr Matthew Cheesman,

School of Biomedical Sciences, University of Queensland, Brisbane Queensland 4072, Australia